Synthesis of glycosylphosphatidylinositol (GPI)-anchor glycolipids bearing unsaturated lipids
نویسندگان
چکیده
منابع مشابه
Defects in glycosylphosphatidylinositol (GPI) anchor synthesis activate Hog1 kinase and confer copper-resistance in Saccharomyces cerevisisae.
Las21/Gpi7 contains a heavy-metal-associated motif at its N-terminus. When this motif was disrupted by amino acid substitution, the cells acquired weak copper-resistance. We found that the previously isolated las21 mutants were strongly resistant to copper. Metallothionein is necessary for the expression of the copper-resistance of the las21 mutants. However, hyper-production of metallothionein...
متن کاملSynthesis of non-hydrolysable mimics of glycosylphosphatidylinositol (GPI) anchors.
Synthesis of first generation non-hydrolysable C-phosphonate GPI analogs, viz., 6-O-(2-amino-2-deoxy-α-d-glucopyranosyl)-d-myo-inositol-1-O-(sn-3,4-bis(palmitoyloxy)butyl-1-phosphonate) and 6-O-(2-amino-2-deoxy-α-d-glucopyranosyl)-d-myo-inositol-1-O-(sn-2,3-bis(palmitoyloxy)propyl-1-phosphonate) 23b, is reported. The target compounds were synthesized by the coupling of α-pseudodisaccharide 21 w...
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Boundary proteomics pecialized regions of chromatin flank the edges of transcriptionally silent DNA, separating it from euchromatin. For example, such boundary elements insulate the silent mating type loci ( HMR and HML ) in yeast. The molecular nature of these boundaries has remained obscure. Now, Tackett et al. (page 35) have identified protein complexes that are important for maintaining suc...
متن کاملSecretion of cryptococcal phospholipase B1 (PLB1) is regulated by a glycosylphosphatidylinositol (GPI) anchor.
The secreted, multifunctional enzyme PLB1 (phospholipase B1 protein encoded by the PLB1 gene) is a virulence determinant of the pathogenic fungus Cryptococcus neoformans, but the mechanism of its secretion is unknown. The cryptococcal PLB1 gene encodes putative, N-terminal LP (leader peptide) and C-terminal GPI (glycosylphosphatidylinositol) anchor attachment motifs, suggesting that PLB1 is GPI...
متن کاملMechanical compressibility of the glycosylphosphatidylinositol (GPI) anchor backbone governed by independent glycosidic linkages.
About 1% of the human proteome is anchored to the outer leaflet of cell membranes via a class of glycolipids called GPI anchors. In spite of their ubiquity, experimental information about the conformational dynamics of these glycolipids is rather limited. Here, we use a variety of computer simulation techniques to elucidate the conformational flexibility of the Man-α(1→2)-Man-α(1→6)-Man-α(1→4)-...
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ژورنال
عنوان ژورنال: Chemical Communications
سال: 2016
ISSN: 1359-7345,1364-548X
DOI: 10.1039/c5cc07694c